Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus

Sara Bahonar; Maryam Ghazvinian; Mohamad Reza Haghshenas; Hamid Reza Goli; Bahman Mirzaei

Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus

Rep Biochem Mol Biol. 2019 Jul;8(2):161-167.

Authors

Sara Bahonar^{1

2}, Maryam Ghazvinian¹, Mohamad Reza Haghshenas¹, Hamid Reza Goli¹, Bahman Mirzaei^{1

2}

Affiliations

¹ Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science.
² Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.

PMID: 31832440
PMCID: PMC6844615

Abstract

Background: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals.

Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host.

Results: LPurification of the macromolecules was confirmed by FTIR and Western blotting.

Conclusion: The S. epidermidis SesC protein and PIA were uccessfully purified. Both are considered as vaccine candidates.

Keywords: Biofilm formation; Polysaccharide Intracellular Adhesin; Staphylococcus aureus.